Hotspot

atpH-atpI

LSC · 13977-14807 · 831 bp

Back to Guihaia

Pi 0

MarkerSeek score 40.00

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 831
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 40.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	ATAACGGAAGCGGCAGAAAT	ATTTTTGCAACGTTAGCCGC	45.0	45.0	58.0	58.6	920 bp	1.0	75.0
atpH-atpI_p2	ATAACGGAAGCGGCAGAAAT	TAGGTGAATCCATGGAGGGT	45.0	50.0	58.0	57.4	892 bp	1.0	75.0
atpH-atpI_p3	TACCTTGACCAACTCCAGGT	ATTTTTGCAACGTTAGCCGC	50.0	45.0	57.9	58.6	976 bp	1.0	74.4
atpH-atpI_p4	TACCTTGACCAACTCCAGGT	TAGGTGAATCCATGGAGGGT	50.0	50.0	57.9	57.4	948 bp	1.0	74.4
atpH-atpI_p5	AATAACGGAAGCGGCAGAAA	ATTTTTGCAACGTTAGCCGC	45.0	45.0	58.2	58.6	921 bp	1.0	73.8