Hotspot

matK-trnK-UUU

LSC · 3345-4164 · 820 bp

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Pi 0

MarkerSeek score 40.00

Primer available yes

Label matK-trnK-UUU

Feature Metadata

label_name: matK-trnK-UUU
feature_type: igs
region: LSC
length_bp: 820
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 40.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
matK-trnK-UUU_p1	ACTCCTTAAAGAGAAGCGGA	AACGGTAGAGTACTCGGCTT	45.0	50.0	55.9	58.2	923 bp	1.0	61.8
matK-trnK-UUU_p2	ACTCCTTAAAGAGAAGCGGA	ACGGTAGAGTACTCGGCTTT	45.0	50.0	55.9	58.2	922 bp	1.0	61.8
matK-trnK-UUU_p3	ACTCCTTAAAGAGAAGCGGA	GTCGTGCAGTGCAATTCAAT	45.0	45.0	55.9	57.7	1013 bp	1.0	60.4
matK-trnK-UUU_p4	ACTCCTTAAAGAGAAGCGGA	GGGTTGCTAACTCAACGGTA	45.0	50.0	55.9	57.5	936 bp	1.0	59.4
matK-trnK-UUU_p5	ACTCCTTAAAGAGAAGCGGA	TTCGGGTTGCTAACTCAACG	45.0	50.0	55.9	58.5	939 bp	1.0	59.1