primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
psbA_p1	psbA	1	GGAGAGCCCCAATTGATACA	GTGTGCTTGGGAGTTCTTGA	20	20	50.0	50.0	57.0	58.0	7.1	0.0	1.062319	210	1271	1141-1141	0.500000	51.5
psbA_p2	psbA	2	GGAGAGCCCCAATTGATACA	ATACGTGTGCTTGGGAGTTC	20	20	50.0	50.0	57.0	57.9	7.1	0.0	1.124400	210	1271	1145-1145	0.500000	51.0
psbA_p3	psbA	3	GGAGAGCCCCAATTGATACAC	GTGTGCTTGGGAGTTCTTGA	21	20	52.4	50.0	58.4	58.0	7.1	0.0	1.456067	210	1271	1141-1141	0.500000	48.4
psbA_p4	psbA	4	GGAGAGCCCCAATTGATACAC	ATACGTGTGCTTGGGAGTTC	21	20	52.4	50.0	58.4	57.9	7.1	0.0	1.518147	210	1271	1145-1145	0.500000	47.9
psbA_p5	psbA	5	GGAGAGCCCCAATTGATACA	AATACGTGTGCTTGGGAGTT	20	20	50.0	45.0	57.0	57.4	7.1	0.0	1.591116	210	1271	1146-1146	0.500000	47.3
matK-trnK-UUU_p1	matK-trnK-UUU	1	ACTCCTTAAAGAGAAGCGGA	AACGGTAGAGTACTCGGCTT	20	20	45.0	50.0	55.9	58.2	8.1	17.1	2.280619	3345	4164	923-923	1.000000	61.8
matK-trnK-UUU_p2	matK-trnK-UUU	2	ACTCCTTAAAGAGAAGCGGA	ACGGTAGAGTACTCGGCTTT	20	20	45.0	50.0	55.9	58.2	8.1	17.1	2.280619	3345	4164	922-922	1.000000	61.8
matK-trnK-UUU_p3	matK-trnK-UUU	3	ACTCCTTAAAGAGAAGCGGA	GTCGTGCAGTGCAATTCAAT	20	20	45.0	45.0	55.9	57.7	8.1	21.7	2.448754	3345	4164	1013-1013	1.000000	60.4
matK-trnK-UUU_p4	matK-trnK-UUU	4	ACTCCTTAAAGAGAAGCGGA	GGGTTGCTAACTCAACGGTA	20	20	45.0	50.0	55.9	57.5	8.1	0.0	2.572956	3345	4164	936-936	1.000000	59.4
matK-trnK-UUU_p5	matK-trnK-UUU	5	ACTCCTTAAAGAGAAGCGGA	TTCGGGTTGCTAACTCAACG	20	20	45.0	50.0	55.9	58.5	8.1	0.0	2.607840	3345	4164	939-939	1.000000	59.1
trnK-UUU-rps16_p1	trnK-UUU-rps16	1	AGTCTGGACGAATCTGTTGC	GCTCAACCTACGGGAACTG	20	19	50.0	57.9	57.9	57.9	0.0	0.0	1.225083	4202	4953	923-923	1.000000	70.2
trnK-UUU-rps16_p2	trnK-UUU-rps16	2	GTCTGGACGAATCTGTTGCT	GCTCAACCTACGGGAACTG	20	19	50.0	57.9	57.9	57.9	0.0	0.0	1.225083	4202	4953	922-922	1.000000	70.2
trnK-UUU-rps16_p3	trnK-UUU-rps16	3	AGTCTGGACGAATCTGTTGC	GTGCTCAACCTACGGGAAC	20	19	50.0	57.9	57.9	58.2	0.0	0.0	1.249110	4202	4953	925-925	1.000000	70.0
trnK-UUU-rps16_p4	trnK-UUU-rps16	4	GTCTGGACGAATCTGTTGCT	GTGCTCAACCTACGGGAAC	20	19	50.0	57.9	57.9	58.2	0.0	0.0	1.249110	4202	4953	924-924	1.000000	70.0
trnK-UUU-rps16_p5	trnK-UUU-rps16	5	AAGCCGAGTACTCTACCGTT	GCTCAACCTACGGGAACTG	20	19	50.0	57.9	58.2	57.9	20.3	0.0	1.307449	4202	4953	866-866	1.000000	69.5
rps16_p1	rps16	1	GCAAGAAAAAGGCAAATTGTGT	CCGGTTGAGCCAATGACTAT	22	20	36.4	50.0	57.1	57.7	0.0	0.0	3.212950	4954	6190	1421-1421	1.000000	54.3
rps16_p2	rps16	2	GCAAGAAAAAGGCAAATTGTGT	TATACACACACCGGTTGAGC	22	20	36.4	50.0	57.1	57.6	0.0	19.4	3.254427	4954	6190	1431-1431	1.000000	54.0
rps16_p3	rps16	3	ACAGCAAGAAAAAGGCAAATTG	CCGGTTGAGCCAATGACTAT	22	20	36.4	50.0	56.9	57.7	0.0	0.0	3.480732	4954	6190	1424-1424	1.000000	52.2
rps16_p4	rps16	4	AGCAAGAAAAAGGCAAATTGTG	CCGGTTGAGCCAATGACTAT	22	20	36.4	50.0	56.9	57.7	0.0	0.0	3.480732	4954	6190	1422-1422	1.000000	52.2
rps16_p5	rps16	5	CAGCAAGAAAAAGGCAAATTGT	CCGGTTGAGCCAATGACTAT	22	20	36.4	50.0	56.9	57.7	0.0	0.0	3.480732	4954	6190	1423-1423	1.000000	52.2
rps16-trnQ-UUG_p1	rps16-trnQ-UUG	1	CAGCCGATCATGTCCTTCAA	GAGGTTCGAATCCTTCCGTC	20	20	50.0	55.0	58.0	58.1	0.0	16.9	0.104521	6191	7315	1216-1216	1.000000	79.2
rps16-trnQ-UUG_p2	rps16-trnQ-UUG	2	GCCGATCATGTCCTTCAAGT	GAGGTTCGAATCCTTCCGTC	20	20	50.0	55.0	58.0	58.1	0.0	16.9	0.105939	6191	7315	1214-1214	1.000000	79.2
rps16-trnQ-UUG_p3	rps16-trnQ-UUG	3	AATCCACAGCCGATCATGTC	GAGGTTCGAATCCTTCCGTC	20	20	50.0	55.0	58.0	58.1	0.0	16.9	0.117059	6191	7315	1222-1222	1.000000	79.1
rps16-trnQ-UUG_p4	rps16-trnQ-UUG	4	GAATCCACAGCCGATCATGT	GAGGTTCGAATCCTTCCGTC	20	20	50.0	55.0	58.0	58.1	0.0	16.9	0.117059	6191	7315	1223-1223	1.000000	79.1
rps16-trnQ-UUG_p5	rps16-trnQ-UUG	5	CAGCCGATCATGTCCTTCAA	GAAATTCGAAATGGGGCGTG	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.120355	6191	7315	1275-1275	1.000000	79.0
trnG-UCC_p1	trnG-UCC	1	ATTGCGCCTCACTCTTTTGT	TGCATATCCAAATTGATGATGCA	20	23	45.0	34.8	58.4	57.2	4.2	18.0	4.183822	9129	9886	889-889	1.000000	46.5
trnG-UCC_p2	trnG-UCC	2	CGCCTCACTCTTTTGTTCGA	TGCATATCCAAATTGATGATGCA	20	23	50.0	34.8	58.5	57.2	0.0	18.0	4.295039	9129	9886	885-885	1.000000	45.6
trnG-UCC_p3	trnG-UCC	3	TTGCGCCTCACTCTTTTGTT	TGCATATCCAAATTGATGATGCA	20	23	45.0	34.8	58.6	57.2	4.2	18.0	4.404483	9129	9886	888-888	1.000000	44.8
trnG-UCC_p4	trnG-UCC	4	ACGAAGTGTCAACGCTAGAA	TGCATATCCAAATTGATGATGCA	20	23	45.0	34.8	57.2	57.2	0.0	18.0	4.580631	9129	9886	934-934	1.000000	43.4
trnG-UCC_p5	trnG-UCC	5	ATTGCGCCTCACTCTTTTGT	TGCATATCCAAATTGATGATGCAT	20	24	45.0	33.3	58.4	57.6	4.2	18.0	4.744197	9129	9886	889-889	1.000000	42.0
atpF_p1	atpF	1	ACTAATTTCGTCGGCTCGAA	AGAACTCTGTTCTTTGTTAGTCCT	20	24	45.0	37.5	57.4	57.5	9.6	7.1	5.129437	11847	13196	1504-1504	1.000000	40.0
atpF_p2	atpF	2	ACTAATTTCGTCGGCTCGAA	AGTCCTATCTATAAGAGGAGAGCA	20	24	45.0	41.7	57.4	57.3	9.6	0.0	5.354252	11847	13196	1486-1486	1.000000	40.0
atpF_p3	atpF	3	GGCTCGAAGGGTTACCATTA	AGAACTCTGTTCTTTGTTAGTCCT	20	24	50.0	37.5	57.0	57.5	0.0	7.1	5.481484	11847	13196	1492-1492	1.000000	40.0
atpF_p4	atpF	4	ACTAATTTCGTCGGCTCGAA	AGAACTCTGTTCTTTGTTAGTCC	20	23	45.0	39.1	57.4	56.2	9.6	7.1	5.482017	11847	13196	1504-1504	1.000000	40.0
atpF_p5	atpF	5	ACTAATTTCGTCGGCTCGAA	GAACTCTGTTCTTTGTTAGTCCT	20	23	45.0	39.1	57.4	56.2	9.6	3.5	5.482017	11847	13196	1503-1503	1.000000	40.0
atpH-atpI_p1	atpH-atpI	1	ATAACGGAAGCGGCAGAAAT	ATTTTTGCAACGTTAGCCGC	20	20	45.0	45.0	58.0	58.6	1.4	16.5	0.624009	13977	14807	920-920	1.000000	75.0
atpH-atpI_p2	atpH-atpI	2	ATAACGGAAGCGGCAGAAAT	TAGGTGAATCCATGGAGGGT	20	20	45.0	50.0	58.0	57.4	1.4	21.3	0.625447	13977	14807	892-892	1.000000	75.0
atpH-atpI_p3	atpH-atpI	3	TACCTTGACCAACTCCAGGT	ATTTTTGCAACGTTAGCCGC	20	20	50.0	45.0	57.9	58.6	0.0	16.5	0.697901	13977	14807	976-976	1.000000	74.4
atpH-atpI_p4	atpH-atpI	4	TACCTTGACCAACTCCAGGT	TAGGTGAATCCATGGAGGGT	20	20	50.0	50.0	57.9	57.4	0.0	21.3	0.699339	13977	14807	948-948	1.000000	74.4
atpH-atpI_p5	atpH-atpI	5	AATAACGGAAGCGGCAGAAA	ATTTTTGCAACGTTAGCCGC	20	20	45.0	45.0	58.2	58.6	1.4	16.5	0.769548	13977	14807	921-921	1.000000	73.8
trnT-UGU-trnL-UAA_p1	trnT-UGU-trnL-UAA	1	GCGATGCTCTAACCTCTGAG	ACCAATTTCGCCATATCCCC	20	20	55.0	50.0	57.9	58.0	0.0	0.0	0.137364	47186	48003	868-1079	1.000000	78.9
trnT-UGU-trnL-UAA_p2	trnT-UGU-trnL-UAA	2	GCGATGCTCTAACCTCTGAG	TAGCGTCTACCAATTTCGCC	20	20	55.0	50.0	57.9	58.1	0.0	0.0	0.202173	47186	48003	876-1087	1.000000	78.4
trnT-UGU-trnL-UAA_p3	trnT-UGU-trnL-UAA	3	GGAATCGAACCGATGACCAT	ACCAATTTCGCCATATCCCC	20	20	50.0	50.0	57.8	58.0	15.5	0.0	0.245117	47186	48003	900-1111	1.000000	78.0
trnT-UGU-trnL-UAA_p4	trnT-UGU-trnL-UAA	4	GGAATCGAACCGATGACCAT	TAGCGTCTACCAATTTCGCC	20	20	50.0	50.0	57.8	58.1	15.5	0.0	0.309926	47186	48003	908-1119	1.000000	77.5
trnT-UGU-trnL-UAA_p5	trnT-UGU-trnL-UAA	5	GCGATGCTCTAACCTCTGAG	ATCCAATCAAGTCCGTAGCG	20	20	55.0	50.0	57.9	57.8	0.0	0.0	0.357808	47186	48003	891-1102	1.000000	77.1
psaC-ndhE_p1	psaC-ndhE	1	ACATCCGTCCCAAGGTATCA	CTGAAGCAGCTATTGGGCTA	20	20	50.0	50.0	58.1	57.4	0.0	0.0	0.753385	120183	121481	1493-1730	1.000000	74.3
psaC-ndhE_p2	psaC-ndhE	2	ACATCCGTCCCAAGGTATCA	TGAAGCAGCTATTGGGCTAG	20	20	50.0	50.0	58.1	57.4	0.0	0.0	0.753385	120183	121481	1492-1729	1.000000	74.3
psaC-ndhE_p3	psaC-ndhE	3	ACATCCGTCCCAAGGTATCA	TGTTATAGCTGTTGCGGCTG	20	20	50.0	50.0	58.1	58.6	0.0	12.2	0.757229	120183	121481	1513-1750	1.000000	74.2
psaC-ndhE_p4	psaC-ndhE	4	ACATCCGTCCCAAGGTATCA	GAAGCAGCTATTGGGCTAGC	20	20	50.0	55.0	58.1	58.8	0.0	6.0	0.891951	120183	121481	1491-1728	1.000000	73.2
psaC-ndhE_p5	psaC-ndhE	5	CAATACATCTGTGGGGCAGG	TGAAGCAGCTATTGGGCTAG	20	20	55.0	50.0	58.3	57.4	0.0	0.0	0.943571	120183	121481	1468-1705	1.000000	72.8
ycf1_p1	ycf1	1	CGCCATTAGATCCGTGTCAT	CTTGCCAGAATTTGTGCCAC	20	20	50.0	50.0	57.8	58.2	0.0	0.0	0.367841	127618	128617	1197-1197	1.000000	77.1
ycf1_p2	ycf1	2	CGCCATTAGATCCGTGTCAT	TCTTGCCAGAATTTGTGCCA	20	20	50.0	45.0	57.8	58.3	0.0	0.0	0.452672	127618	128617	1198-1198	1.000000	76.4
ycf1_p3	ycf1	3	CGCCATTAGATCCGTGTCAT	CCGGTTCTTGCCAGAATTTG	20	20	50.0	50.0	57.8	57.6	0.0	3.2	0.507357	127618	128617	1203-1203	1.000000	75.9
ycf1_p4	ycf1	4	CGCCATTAGATCCGTGTCAT	GCCAGAATTTGTGCCACTTT	20	20	50.0	45.0	57.8	57.5	0.0	0.0	0.686376	127618	128617	1194-1194	1.000000	74.5
ycf1_p5	ycf1	5	CGCCATTAGATCCGTGTCAT	TGCCAGAATTTGTGCCACTT	20	20	50.0	45.0	57.8	58.6	0.0	0.0	0.742775	127618	128617	1195-1195	1.000000	74.1