Hotspot

trnN-GUU-trnR-ACG

IRa · 135109-135806 · 698 bp

Back to Guibourtia

Pi 0.0248

MarkerSeek score 79.10

Primer available yes

Label trnN-GUU-trnR-ACG

Feature Metadata

label_name: trnN-GUU-trnR-ACG
feature_type: igs
region: IRa
length_bp: 698
variable_sites: 31
pi: 0.0248
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 79.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnN-GUU-trnR-ACG_p1	TGGGTTGAGAACGGGAATTG	CAGAGGATTAGAGCACGTGG	50.0	55.0	58.1	57.8	762-906 bp	1.0	84.5
trnN-GUU-trnR-ACG_p2	GCTGTTAACTGACTGGTCGT	CAGAGGATTAGAGCACGTGG	50.0	55.0	57.8	57.8	685-829 bp	1.0	84.3
trnN-GUU-trnR-ACG_p3	TGGTCGTAGGTTCGAATCCT	CAGAGGATTAGAGCACGTGG	50.0	55.0	58.2	57.8	672-816 bp	1.0	84.3
trnN-GUU-trnR-ACG_p4	TGGGTTGAGAACGGGAATTG	GAACAGATCCAGTGGAGACG	50.0	55.0	58.1	57.7	799-943 bp	1.0	83.8
trnN-GUU-trnR-ACG_p5	GGGTTGAGAACGGGAATTGA	CAGAGGATTAGAGCACGTGG	50.0	55.0	57.8	57.8	761-905 bp	1.0	83.7