Hotspot

rbcL-accD

LSC · 60510-61410 · 901 bp

Back to Guibourtia

Pi 0.038

MarkerSeek score 75.60

Primer available yes

Label rbcL-accD

Feature Metadata

label_name: rbcL-accD
feature_type: igs
region: LSC
length_bp: 901
variable_sites: 56
pi: 0.038
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 75.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-accD_p1	AGTCCTGAATTAGCTGCTGC	TTAGTTAGCCCACACCCGTA	50.0	50.0	58.0	58.1	1044-1138 bp	1.0	87.3
rbcL-accD_p2	GAGGCTAGCAAATGGAGTCC	TTAGTTAGCCCACACCCGTA	55.0	50.0	58.0	58.1	1059-1153 bp	1.0	87.2
rbcL-accD_p3	AGTCCTGAATTAGCTGCTGC	TAGTTAGCCCACACCCGTAT	50.0	50.0	58.0	57.8	1043-1137 bp	1.0	86.5
rbcL-accD_p4	GAGGCTAGCAAATGGAGTCC	TAGTTAGCCCACACCCGTAT	55.0	50.0	58.0	57.8	1058-1152 bp	1.0	86.4
rbcL-accD_p5	AGTCCTGAATTAGCTGCTGC	GCCCACACCCGTATTCTAAC	50.0	55.0	58.0	58.3	1037-1131 bp	1.0	85.1