Hotspot

ndhC-trnV-UAC

LSC · 54715-55196 · 482 bp

Back to Guibourtia

Pi 0.0396
MarkerSeek score 74.80
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
482
variable_sites
34
pi
0.0396
unique_haplotype_count
5
primer_available
yes
alignment_reliability
0.98
markerseek_score
74.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 CATCATATTCATAAAGCAAAAACA GTTCGAGTCCGTATAGCCCT 25.0 55.0 52.7 58.7 464-543 bp 1.0 49.4
ndhC-trnV-UAC_p2 CATCATATTCATAAAGCAAAAACA GAAGGTCTACGGTTCGAGTC 25.0 55.0 52.7 57.5 475-554 bp 1.0 49.3
ndhC-trnV-UAC_p3 CATCATATTCATAAAGCAAAAACA AGAAGGTCTACGGTTCGAGT 25.0 50.0 52.7 57.8 476-555 bp 1.0 49.2
ndhC-trnV-UAC_p4 ACATCATATTCATAAAGCAAAAAC AGAAGGTCTACGGTTCGAGT 25.0 50.0 52.7 57.8 477-556 bp 1.0 49.2
ndhC-trnV-UAC_p5 ACATCATATTCATAAAGCAAAAAC GAAGGTCTACGGTTCGAGTC 25.0 55.0 52.7 57.5 476-555 bp 1.0 49.2