Hotspot

psbC-trnS-UGA

LSC · 38467-38699 · 233 bp

Back to Guibourtia

Pi 0.0458

MarkerSeek score 79.00

Primer available yes

Label psbC-trnS-UGA

Feature Metadata

label_name: psbC-trnS-UGA
feature_type: igs
region: LSC
length_bp: 233
variable_sites: 20
pi: 0.0458
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 79.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbC-trnS-UGA_p1	AGCTGCAGCAGGATTTGAAA	GAGAGATGGCTGAGTGGTTG	45.0	55.0	58.4	58.0	394-400 bp	1.0	82.1
psbC-trnS-UGA_p2	AGCTGCAGCAGGATTTGAAA	GGTTGATAGCTCCGGTCTTG	45.0	55.0	58.4	58.1	379-385 bp	1.0	82.0
psbC-trnS-UGA_p3	AGCTGCAGCAGGATTTGAAA	GCTGAGTGGTTGATAGCTCC	45.0	55.0	58.4	58.1	386-392 bp	1.0	81.9
psbC-trnS-UGA_p4	AGCTGCAGCAGGATTTGAAA	GGCTGAGTGGTTGATAGCTC	45.0	55.0	58.4	58.1	387-393 bp	1.0	81.9
psbC-trnS-UGA_p5	TAGGTCATTTATGGCACGCG	GAGAGATGGCTGAGTGGTTG	50.0	55.0	58.4	58.0	428-434 bp	1.0	81.3