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trnC-GCA-petN

LSC · 29709-30618 · 910 bp

Back to Guibourtia

Pi 0.0749

MarkerSeek score 70.50

Primer available yes

Label trnC-GCA-petN

Feature Metadata

label_name: trnC-GCA-petN
feature_type: igs
region: LSC
length_bp: 910
variable_sites: 105
pi: 0.0749
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.83
markerseek_score: 70.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-petN_p1	ACGCACGATAATTTCCCGAA	AGCCCAAGCGAGACTTACTA	45.0	50.0	58.0	58.2	1173-1378 bp	1.0	90.4
trnC-GCA-petN_p2	TTCGTTCGGGACAGCATATG	AGCCCAAGCGAGACTTACTA	50.0	50.0	58.1	58.2	1248-1453 bp	1.0	89.7
trnC-GCA-petN_p3	ACGCACGATAATTTCCCGAA	AACCTTTTCCCTCCCTCCTT	45.0	50.0	58.0	58.2	1533-1763 bp	1.0	89.5
trnC-GCA-petN_p4	ACGCACGATAATTTCCCGAA	ACCTTTTCCCTCCCTCCTTT	45.0	50.0	58.0	58.2	1532-1762 bp	1.0	89.5
trnC-GCA-petN_p5	TTCGTTCGGGACAGCATATG	ACCTTTTCCCTCCCTCCTTT	50.0	50.0	58.1	58.2	1607-1837 bp	1.0	88.8