Hotspot

atpH-atpI

LSC · 14729-16134 · 1406 bp

Back to Guibourtia

Pi 0.0333

MarkerSeek score 70.80

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 1406
variable_sites: 83
pi: 0.0333
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 70.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	AATAGAAGCAAGCCCGACAG	TATGGTCCATGGGTTCCCTT	50.0	50.0	58.0	58.0	1763-2064 bp	1.0	87.4
atpH-atpI_p2	ATAACGGAAGCGGCAGAAAT	TATGGTCCATGGGTTCCCTT	45.0	50.0	58.0	58.0	1729-2030 bp	1.0	87.4
atpH-atpI_p3	AGGAAGAAGGCGAGTCGATA	TATGGTCCATGGGTTCCCTT	50.0	50.0	57.9	58.0	2128-2417 bp	1.0	86.9
atpH-atpI_p4	AAGCGCTAATGCTACAACCA	TATGGTCCATGGGTTCCCTT	45.0	50.0	57.9	58.0	1919-2220 bp	1.0	86.5
atpH-atpI_p5	GGGCTCGTTGGACTAACAAT	TATGGTCCATGGGTTCCCTT	50.0	50.0	57.9	58.0	2164-2453 bp	1.0	86.4