Hotspot

psbB

LSC · 71013-72539 · 1527 bp

Back to Guadua

Pi 0.000873
MarkerSeek score 59.30
Primer available yes
Label psbB

Feature Metadata

label_name
psbB
feature_type
gene
region
LSC
length_bp
1527
variable_sites
2
pi
0.000873
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbB_p1 TGACACAGAATCCCCTAGAGG AGAAAGGATGGGAGATGTTTCC 52.4 45.5 58.2 57.8 1708 bp 1.0 52.2
psbB_p2 TGACACAGAATCCCCTAGAGG GAGTCAAAGAAAGGATGGGAGA 52.4 45.5 58.2 57.4 1715 bp 1.0 49.6
psbB_p3 TGACACAGAATCCCCTAGAGG AGAGTCAAAGAAAGGATGGGAG 52.4 45.5 58.2 57.4 1716 bp 1.0 49.6
psbB_p4 GACACAGAATCCCCTAGAGG AGAAAGGATGGGAGATGTTTCC 55.0 45.5 56.4 57.8 1707 bp 1.0 49.5
psbB_p5 TGACACAGAATCCCCTAGAGG GGAGATGTTTCCCATGTCAA 52.4 45.0 58.2 55.3 1698 bp 1.0 48.7