Hotspot

ndhF

SSC · 102748-104967 · 2220 bp

Back to Guadua

Pi 0.0003
MarkerSeek score 38.30
Primer available yes
Label ndhF

Feature Metadata

label_name
ndhF
feature_type
gene
region
SSC
length_bp
2220
variable_sites
1
pi
0.0003
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
38.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF_p1 GGGGGAGAGATGGTAGAAGG TGGTTTGGTGAATCGGAAACA 60.0 42.9 58.3 58.3 2365-2382 bp 1.0 67.1
ndhF_p2 AGAAAAGGGGGAGAGATGGT TGGTTTGGTGAATCGGAAACA 50.0 42.9 57.6 58.3 2371-2388 bp 1.0 66.3
ndhF_p3 GGGGGAGAGATGGTAGAAGG GGTTTGGTGAATCGGAAACA 60.0 45.0 58.3 56.5 2364-2381 bp 1.0 66.2
ndhF_p4 GGGGGAGAGATGGTAGAAGG TGGTTTGGTGAATCGGAAAC 60.0 45.0 58.3 56.5 2365-2382 bp 1.0 66.2
ndhF_p5 AGAAAAGGGGGAGAGATGGT GGTTTGGTGAATCGGAAACA 50.0 45.0 57.6 56.5 2370-2387 bp 1.0 65.4