Hotspot

petA-psbJ

LSC · 62664-63651 · 988 bp

Back to Guadua

Pi 0.00135
MarkerSeek score 59.60
Primer available yes
Label petA-psbJ

Feature Metadata

label_name
petA-psbJ
feature_type
igs
region
LSC
length_bp
988
variable_sites
2
pi
0.00135
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petA-psbJ_p1 GCGTCCAAGGCCTTTTATTC TCCTCTTTGGCTGATAGGTACT 50.0 45.5 57.4 58.0 1198 bp 1.0 59.6
petA-psbJ_p2 GCGTCCAAGGCCTTTTATTC CCTCTTTGGCTGATAGGTACTG 50.0 50.0 57.4 57.9 1197 bp 1.0 59.1
petA-psbJ_p3 GCGTCCAAGGCCTTTTATTC TTCCTCTTTGGCTGATAGGT 50.0 45.0 57.4 55.8 1199 bp 1.0 58.2
petA-psbJ_p4 GCGTCCAAGGCCTTTTATTC ACGGTTCATATTCTGGATTGGG 50.0 45.5 57.4 58.2 1133 bp 1.0 58.2
petA-psbJ_p5 GCGTCCAAGGCCTTTTATTC CGGTTCATATTCTGGATTGGGT 50.0 45.5 57.4 58.2 1132 bp 1.0 58.2