Hotspot

matK-trnK-UUU

LSC · 3236-3920 · 685 bp

Back to Guadua

Pi 0.000975
MarkerSeek score 59.50
Primer available yes
Label matK-trnK-UUU

Feature Metadata

label_name
matK-trnK-UUU
feature_type
igs
region
LSC
length_bp
685
variable_sites
1
pi
0.000975
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
matK-trnK-UUU_p1 ACTCCTGAAAGAGAAGTGGA CGTGCGGTGTAATTCCATTG 45.0 50.0 55.4 57.8 861-862 bp 1.0 58.0
matK-trnK-UUU_p2 ACTCCTGAAAGAGAAGTGGAT CGTGCGGTGTAATTCCATTG 42.9 50.0 56.0 57.8 861-862 bp 1.0 54.7
matK-trnK-UUU_p3 CATAATCATGGGCAAATGCA CGTGCGGTGTAATTCCATTG 40.0 50.0 54.6 57.8 888-889 bp 1.0 52.0
matK-trnK-UUU_p4 ACTCCTGAAAGAGAAGTGGA TGGGTTGCTAACTCAATGGT 45.0 45.0 55.4 57.0 802-803 bp 1.0 51.6
matK-trnK-UUU_p5 ACTCCTGAAAGAGAAGTGGA TCAATGGTAGAGTACTCGGCT 45.0 47.6 55.4 58.0 790-791 bp 1.0 51.3