Hotspot

ndhA

SSC · 120784-122981 · 2198 bp

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Pi 0.0169

MarkerSeek score 65.80

Primer available yes

Label ndhA

Feature Metadata

label_name: ndhA
feature_type: gene
region: SSC
length_bp: 2198
variable_sites: 37
pi: 0.0169
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhA_p1	ACCCAGTTACCATAGGGAACA	GCCCACCGGGTTTTATCAAT	47.6	50.0	58.1	58.5	2405-2415 bp	1.0	68.8
ndhA_p2	TGAACCCAGTTACCATAGGGA	GCCCACCGGGTTTTATCAAT	47.6	50.0	57.8	58.5	2408-2418 bp	1.0	67.7
ndhA_p3	ACCCAGTTACCATAGGGAACA	GCCCACCGGGTTTTATCAA	47.6	52.6	58.1	58.0	2405-2415 bp	1.0	64.7
ndhA_p4	TGAACCCAGTTACCATAGGGA	GCCCACCGGGTTTTATCAA	47.6	52.6	57.8	58.0	2408-2418 bp	1.0	63.6
ndhA_p5	CCCAGTTACCATAGGGAACA	GCCCACCGGGTTTTATCAAT	50.0	50.0	56.2	58.5	2404-2414 bp	1.0	63.1