Hotspot

ccsA

SSC · 115430-116392 · 963 bp

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Pi 0.00623

MarkerSeek score 49.60

Primer available yes

Label ccsA

Feature Metadata

label_name: ccsA
feature_type: gene
region: SSC
length_bp: 963
variable_sites: 6
pi: 0.00623
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 49.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA_p1	ATAGGTAGACACGCTGCTCT	CTCGACGAAATGGGTGGAAT	50.0	50.0	57.9	58.0	1815-1820 bp	1.0	81.0
ccsA_p2	TAGACACGCTGCTCTTAGGA	CTCGACGAAATGGGTGGAAT	50.0	50.0	57.9	58.0	1810-1815 bp	1.0	80.5
ccsA_p3	ATAGGTAGACACGCTGCTCT	AAATGGTTCTCGCCGAAGTT	50.0	45.0	57.9	58.1	1201 bp	1.0	79.3
ccsA_p4	ATAGGTAGACACGCTGCTCT	AATGGTTCTCGCCGAAGTTT	50.0	45.0	57.9	58.1	1200 bp	1.0	79.3
ccsA_p5	ATAGGTAGACACGCTGCTCT	ATGGTTCTCGCCGAAGTTTT	50.0	45.0	57.9	58.1	1199 bp	1.0	79.2