Hotspot

ndhD-psaC

SSC · 118165-118300 · 136 bp

Back to Grubbia

Pi 0.0308

MarkerSeek score 66.60

Primer available yes

Label ndhD-psaC

Feature Metadata

label_name: ndhD-psaC
feature_type: igs
region: SSC
length_bp: 136
variable_sites: 4
pi: 0.0308
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 66.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD-psaC_p1	AACCCGCAGATATTGGCAAA	AGAGGACTGTGTCGGTTGTA	45.0	50.0	58.2	58.0	297-303 bp	1.0	81.4
ndhD-psaC_p2	ACCCGCAGATATTGGCAAAA	AGAGGACTGTGTCGGTTGTA	45.0	50.0	58.2	58.0	296-302 bp	1.0	81.4
ndhD-psaC_p3	CCCGCAGATATTGGCAAAAC	AGAGGACTGTGTCGGTTGTA	50.0	50.0	57.8	58.0	295-301 bp	1.0	80.9
ndhD-psaC_p4	AAGAACCCGCAGATATTGGC	AGAGGACTGTGTCGGTTGTA	50.0	50.0	58.3	58.0	300-306 bp	1.0	80.0
ndhD-psaC_p5	ACCCGCAGATATTGGCAAAA	AAGAGATGTGAATCCGCCTG	45.0	50.0	58.2	57.7	277-283 bp	1.0	79.0