Hotspot

rpl32-trnL-UAG

SSC · 114377-115250 · 874 bp

Back to Grubbia

Pi 0.0288

MarkerSeek score 71.30

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 874
variable_sites: 25
pi: 0.0288
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 71.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	AAGAAGGGATATTGGGCAGC	AGAGCAGCGTGTCTACCTAT	50.0	50.0	57.6	57.9	1007-1078 bp	1.0	79.0
rpl32-trnL-UAG_p2	GGATATTGGGCAGCGCTAAA	AGAGCAGCGTGTCTACCTAT	50.0	50.0	58.4	57.9	1001-1072 bp	1.0	78.8
rpl32-trnL-UAG_p3	AAGAAGGGATATTGGGCAGC	TCCTAAGAGCAGCGTGTCTA	50.0	50.0	57.6	57.9	1012-1083 bp	1.0	78.5
rpl32-trnL-UAG_p4	GGATATTGGGCAGCGCTAAA	TCCTAAGAGCAGCGTGTCTA	50.0	50.0	58.4	57.9	1006-1077 bp	1.0	78.3
rpl32-trnL-UAG_p5	AAGAAGGGATATTGGGCAGC	AAGAGCAGCGTGTCTACCTA	50.0	50.0	57.6	58.2	1008-1079 bp	1.0	78.2