Hotspot

ndhF-rpl32

SSC · 113520-114214 · 695 bp

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Pi 0.0273

MarkerSeek score 60.40

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 695
variable_sites: 19
pi: 0.0273
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	CCGCAACAAGTCGTGTAAAC	TTGGGTTGAAAGCGGAAAGT	50.0	45.0	58.0	58.2	1878-1892 bp	1.0	79.8
ndhF-rpl32_p2	CGCAACAAGTCGTGTAAACC	TTGGGTTGAAAGCGGAAAGT	50.0	45.0	58.0	58.2	1877-1891 bp	1.0	79.8
ndhF-rpl32_p3	CCGCAACAAGTCGTGTAAAC	CGGAAAGTCCCTGAGGAGTA	50.0	55.0	58.0	58.2	1866 bp	0.5	58.7
ndhF-rpl32_p4	CGCAACAAGTCGTGTAAACC	CGGAAAGTCCCTGAGGAGTA	50.0	55.0	58.0	58.2	1865 bp	0.5	58.7
ndhF-rpl32_p5	ATCGACCAAACAGCCCAATT	CGGAAAGTCCCTGAGGAGTA	45.0	55.0	58.1	58.2	1622 bp	0.5	58.2