Hotspot

psaJ-rpl33

LSC · 68150-68582 · 433 bp

Back to Grubbia

Pi 0.037

MarkerSeek score 69.10

Primer available yes

Label psaJ-rpl33

Feature Metadata

label_name: psaJ-rpl33
feature_type: igs
region: LSC
length_bp: 433
variable_sites: 16
pi: 0.037
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 69.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaJ-rpl33_p1	GGATGCGTTAACATTCCCCT	AATGCCCGTTGATGCCTTAT	50.0	45.0	57.9	57.9	562-563 bp	1.0	81.4
psaJ-rpl33_p2	GGATGCGTTAACATTCCCCT	ATGCCCGTTGATGCCTTATT	50.0	45.0	57.9	57.9	561-562 bp	1.0	81.4
psaJ-rpl33_p3	TTTTCCCGGATGCGTTAACA	AATGCCCGTTGATGCCTTAT	45.0	45.0	58.1	57.9	569-570 bp	1.0	81.0
psaJ-rpl33_p4	TTTTCCCGGATGCGTTAACA	ATGCCCGTTGATGCCTTATT	45.0	45.0	58.1	57.9	568-569 bp	1.0	81.0
psaJ-rpl33_p5	TTCCCGGATGCGTTAACATT	ATGCCCGTTGATGCCTTATT	45.0	45.0	57.9	57.9	566-567 bp	1.0	80.9