Hotspot

trnP-GGG-psaJ

LSC · 67626-68014 · 389 bp

Back to Grubbia

Pi 0.0334

MarkerSeek score 70.60

Primer available yes

Label trnP-GGG-psaJ

Feature Metadata

label_name: trnP-GGG-psaJ
feature_type: igs
region: LSC
length_bp: 389
variable_sites: 13
pi: 0.0334
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 70.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnP-GGG-psaJ_p1	ACCCGTGACATTTTGTACCC	TGTTAACGCATCCGGGAAAA	50.0	45.0	58.1	58.1	557-567 bp	1.0	80.2
trnP-GGG-psaJ_p2	CCCGTGACATTTTGTACCCA	TGTTAACGCATCCGGGAAAA	50.0	45.0	58.1	58.1	556-566 bp	1.0	80.2
trnP-GGG-psaJ_p3	ACAGGATTTGAACCCGTGAC	TGTTAACGCATCCGGGAAAA	50.0	45.0	58.1	58.1	568-578 bp	1.0	80.1
trnP-GGG-psaJ_p4	CAGGATTTGAACCCGTGACA	TGTTAACGCATCCGGGAAAA	50.0	45.0	58.1	58.1	567-577 bp	1.0	80.1
trnP-GGG-psaJ_p5	TGACAGGATTTGAACCCGTG	TGTTAACGCATCCGGGAAAA	50.0	45.0	58.1	58.1	570-580 bp	1.0	80.1