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petA-psbJ

LSC · 64069-64790 · 722 bp

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Pi 0.0152

MarkerSeek score 67.50

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 722
variable_sites: 11
pi: 0.0152
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 67.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	ATTGCGTGTCCAAGGTCTTT	ATTCCGGATTGGGTTCATCC	45.0	50.0	58.0	57.6	863-1151 bp	1.0	77.8
petA-psbJ_p2	TTGCGTGTCCAAGGTCTTTT	ATTCCGGATTGGGTTCATCC	45.0	50.0	58.2	57.6	862-1150 bp	1.0	76.0
petA-psbJ_p3	CATTGCGTGTCCAAGGTCTT	ATTCCGGATTGGGTTCATCC	50.0	50.0	58.8	57.6	864-1152 bp	1.0	71.9
petA-psbJ_p4	TGGCATCTGTTATTCTGGCA	ATTCCGGATTGGGTTCATCC	45.0	50.0	57.2	57.6	834-1122 bp	1.0	71.5
petA-psbJ_p5	ATTGCGTGTCCAAGGTCTTT	TGGTTCATATTCCGGATTGGG	45.0	47.6	58.0	57.8	871-1159 bp	1.0	70.9