Hotspot

ycf3-trnS-GGA

LSC · 45966-46628 · 663 bp

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Pi 0.0121

MarkerSeek score 66.00

Primer available yes

Label ycf3-trnS-GGA

Feature Metadata

label_name: ycf3-trnS-GGA
feature_type: igs
region: LSC
length_bp: 663
variable_sites: 8
pi: 0.0121
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 66.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf3-trnS-GGA_p1	CATTTATCCGCGATCTAGGCA	GCGAAGGTACGGAAAGAGAG	47.6	55.0	58.0	58.1	781-782 bp	1.0	72.0
ycf3-trnS-GGA_p2	CATTTATCCGCGATCTAGGCA	CCCTCGGTAAACAAAAGCCT	47.6	50.0	58.0	58.1	752-753 bp	1.0	72.0
ycf3-trnS-GGA_p3	CATTTATCCGCGATCTAGGCA	ACGGAAAGAGAGGGATTCGA	47.6	50.0	58.0	58.2	773-774 bp	1.0	71.5
ycf3-trnS-GGA_p4	CATTTATCCGCGATCTAGGCA	TAGGCGAAGGTACGGAAAGA	47.6	50.0	58.0	58.2	784-785 bp	1.0	71.4
ycf3-trnS-GGA_p5	CATTTATCCGCGATCTAGGCA	TTCCAATGCTACGCCTTGAA	47.6	45.0	58.0	57.8	723-724 bp	1.0	71.2