Hotspot

petN-psbM

LSC · 29907-31066 · 1160 bp

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Pi 0.0201

MarkerSeek score 56.90

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 1160
variable_sites: 23
pi: 0.0201
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 56.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	AATCGGGAGACGGTACAGAT	TGGGAAGGTGGTCAGATGAT	50.0	50.0	57.9	58.0	2083-2089 bp	1.0	81.5
petN-psbM_p2	ATCGGGAGACGGTACAGATT	TGGGAAGGTGGTCAGATGAT	50.0	50.0	57.9	58.0	2082-2088 bp	1.0	81.5
petN-psbM_p3	AATCGGGAGACGGTACAGAT	GATCGAACAAGGATCCCACC	50.0	55.0	57.9	58.0	2366-2372 bp	1.0	81.1
petN-psbM_p4	ATCGGGAGACGGTACAGATT	GATCGAACAAGGATCCCACC	50.0	55.0	57.9	58.0	2365-2371 bp	1.0	81.1
petN-psbM_p5	TAGTAAGTCTCGCTTGGGCT	TGGGAAGGTGGTCAGATGAT	50.0	50.0	58.2	58.0	1691-1711 bp	1.0	80.5