Hotspot

rps16-trnQ-UUG

LSC · 6251-7836 · 1586 bp

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Pi 0.0184

MarkerSeek score 63.00

Primer available yes

Label rps16-trnQ-UUG

Feature Metadata

label_name: rps16-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 1586
variable_sites: 29
pi: 0.0184
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 63.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	AACGGATCGTGTCCTTCAAG	GAGGTTCGAATCCTTCCGTC	50.0	55.0	57.9	58.1	1676-1681 bp	1.0	80.7
rps16-trnQ-UUG_p2	CCCGTTGCTTTCTACCACAT	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.2	58.1	1653-1658 bp	1.0	79.9
rps16-trnQ-UUG_p3	CAACGGATCGTGTCCTTCAA	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.2	58.1	1677-1682 bp	1.0	79.6
rps16-trnQ-UUG_p4	CGGATCGTGTCCTTCAAGTC	GAGGTTCGAATCCTTCCGTC	55.0	55.0	58.4	58.1	1674-1679 bp	1.0	78.3
rps16-trnQ-UUG_p5	GGATCGTGTCCTTCAAGTCG	GAGGTTCGAATCCTTCCGTC	55.0	55.0	58.4	58.1	1673-1678 bp	1.0	78.3