Hotspot

ycf4

LSC · 58415-59019 · 605 bp

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Pi 0.0283
MarkerSeek score 62.60
Primer available yes
Label ycf4

Feature Metadata

label_name
ycf4
feature_type
gene
region
LSC
length_bp
605
variable_sites
17
pi
0.0283
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
62.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf4_p1 AGGGCTTCCTCTATGAAGAA TCACTGTCAATTCCAATTTCGA 45.0 36.4 55.2 56.3 741-746 bp 1.0 42.4
ycf4_p2 AGGGCTTCCTCTATGAAGAAA TCACTGTCAATTCCAATTTCGA 42.9 36.4 56.0 56.3 741-746 bp 1.0 42.4
ycf4_p3 AGGGCTTCCTCTATGAAGAA TCACTGTCAATTCCAATTTCGAA 45.0 34.8 55.2 57.0 741-746 bp 1.0 42.4
ycf4_p4 AGGGCTTCCTCTATGAAGAAA TCACTGTCAATTCCAATTTCGAA 42.9 34.8 56.0 57.0 741-746 bp 1.0 42.4
ycf4_p5 AGGGCTTCCTCTATGAAGAA CACTGTCAATTCCAATTTCGA 45.0 38.1 55.2 54.8 740-745 bp 1.0 42.4