Hotspot

rpoA

LSC · 76404-77405 · 1002 bp

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Pi 0.00499
MarkerSeek score 61.50
Primer available yes
Label rpoA

Feature Metadata

label_name
rpoA
feature_type
gene
region
LSC
length_bp
1002
variable_sites
5
pi
0.00499
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
61.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpoA_p1 TGGTCTAAAGATTTCAATCACAGA TGGGTGTAGGTCTCCTAAAA 33.3 45.0 56.1 55.1 1204-1205 bp 1.0 40.4
rpoA_p2 TGGTCTAAAGATTTCAATCACAGA TGGGTGTAGGTCTCCTAAAAA 33.3 42.9 56.1 55.9 1204-1205 bp 1.0 40.4
rpoA_p3 TGGTCTAAAGATTTCAATCACAGA AATGGGTGTAGGTCTCCTAA 33.3 45.0 56.1 54.8 1206-1207 bp 1.0 40.4
rpoA_p4 TGGTCTAAAGATTTCAATCACAGA ATGGGTGTAGGTCTCCTAAA 33.3 45.0 56.1 54.8 1205-1206 bp 1.0 40.4
rpoA_p5 TTGGTCTAAAGATTTCAATCACAGA TGGGTGTAGGTCTCCTAAAA 32.0 45.0 56.7 55.1 1205-1206 bp 1.0 40.4