Hotspot

psbT-psbN

LSC · 72672-72727 · 56 bp

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Pi 0.0536
MarkerSeek score 81.70
Primer available yes
Label psbT-psbN

Feature Metadata

label_name
psbT-psbN
feature_type
igs
region
LSC
length_bp
56
variable_sites
3
pi
0.0536
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
81.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbT-psbN_p1 ACCTCCTAAAGTTCCAACGA GGCAACCCTCTCAACAACTA 45.0 50.0 56.1 57.4 140 bp 1.0 62.0
psbT-psbN_p2 GAACCTCCTAAAGTTCCAACGA GGCAACCCTCTCAACAACTA 45.5 50.0 58.0 57.4 142 bp 1.0 61.2
psbT-psbN_p3 AGAACCTCCTAAAGTTCCAACG GGCAACCCTCTCAACAACTA 45.5 50.0 58.0 57.4 143 bp 1.0 61.2
psbT-psbN_p4 AACCTCCTAAAGTTCCAACGA GGCAACCCTCTCAACAACTA 42.9 50.0 56.9 57.4 141 bp 1.0 60.2
psbT-psbN_p5 ACCTCCTAAAGTTCCAACGAA GGCAACCCTCTCAACAACTA 42.9 50.0 56.9 57.4 140 bp 1.0 60.2