Hotspot

rpl20-rps12

LSC · 67295-68097 · 803 bp

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Pi 0.00499
MarkerSeek score 61.70
Primer available yes
Label rpl20-rps12

Feature Metadata

label_name
rpl20-rps12
feature_type
igs
region
LSC
length_bp
803
variable_sites
4
pi
0.00499
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
61.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl20-rps12_p1 TTTTGTCCTCCGTTTCCGAG CAGCCAATTCGAAACGTCAC 50.0 50.0 58.1 58.0 929-930 bp 1.0 79.0
rpl20-rps12_p2 TTTTGTCCTCCGTTTCCGAG AGACAGCCAATTCGAAACGT 50.0 45.0 58.1 58.1 932-933 bp 1.0 78.5
rpl20-rps12_p3 TTTTGTCCTCCGTTTCCGAG ACAAAATCCCCAGCTCTTCG 50.0 50.0 58.1 58.2 911-912 bp 1.0 78.0
rpl20-rps12_p4 AAGTCTTGAATGAGCTCCGC CAGCCAATTCGAAACGTCAC 50.0 50.0 58.3 58.0 974-975 bp 1.0 77.9
rpl20-rps12_p5 TTTTGTCCTCCGTTTCCGAG GTCACAAAATCCCCAGCTCT 50.0 50.0 58.1 57.8 914-915 bp 1.0 77.8