Hotspot

psbE-petL

LSC · 62925-64232 · 1308 bp

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Pi 0.0092
MarkerSeek score 64.30
Primer available yes
Label psbE-petL

Feature Metadata

label_name
psbE-petL
feature_type
igs
region
LSC
length_bp
1308
variable_sites
12
pi
0.0092
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
64.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbE-petL_p1 AGAACGTTCTCCTGTGCTTC AGACCAATAAATAAAGCGGAGGT 50.0 39.1 57.8 57.9 1409-1412 bp 1.0 54.7
psbE-petL_p2 GTTCTCCTGTGCTTCCAGAC AGACCAATAAATAAAGCGGAGGT 55.0 39.1 58.2 57.9 1404-1407 bp 1.0 54.4
psbE-petL_p3 CCTGCAATGAATAGGGAGGG AGACCAATAAATAAAGCGGAGGT 55.0 39.1 57.7 57.9 1483-1486 bp 1.0 53.6
psbE-petL_p4 TCTCCTGTGCTTCCAGACAT AGACCAATAAATAAAGCGGAGGT 50.0 39.1 58.4 57.9 1402-1405 bp 1.0 53.2
psbE-petL_p5 AGAACGTTCTCCTGTGCTTC CAGACCAATAAATAAAGCGGAGG 50.0 43.5 57.8 57.6 1410-1413 bp 1.0 52.2