Hotspot

petA-psbJ

LSC · 61199-62127 · 929 bp

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Pi 0.0121
MarkerSeek score 58.80
Primer available yes
Label petA-psbJ

Feature Metadata

label_name
petA-psbJ
feature_type
igs
region
LSC
length_bp
929
variable_sites
11
pi
0.0121
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.98
markerseek_score
58.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petA-psbJ_p1 TGAGAAGGTTCAATTGTCCGA CATATTCCGGATTGGGCTCA 42.9 50.0 57.2 57.4 974-992 bp 1.0 63.7
petA-psbJ_p2 TGAGAAGGTTCAATTGTCCGA TATTCCGGATTGGGCTCATC 42.9 50.0 57.2 57.1 972-990 bp 1.0 61.4
petA-psbJ_p3 TGAGAAGGTTCAATTGTCCGA TCATATTCCGGATTGGGCTC 42.9 50.0 57.2 57.1 975-993 bp 1.0 61.4
petA-psbJ_p4 TGAGAAGGTTCAATTGTCCGAA CATATTCCGGATTGGGCTCA 40.9 50.0 57.9 57.4 974-992 bp 1.0 61.2
petA-psbJ_p5 TTGAGAAGGTTCAATTGTCCGA CATATTCCGGATTGGGCTCA 40.9 50.0 57.9 57.4 975-993 bp 1.0 61.2