Hotspot

rps16-accD

LSC · 55811-56334 · 524 bp

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Pi 0.0174
MarkerSeek score 64.80
Primer available yes
Label rps16-accD

Feature Metadata

label_name
rps16-accD
feature_type
igs
region
LSC
length_bp
524
variable_sites
9
pi
0.0174
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
64.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-accD_p1 AACAAGGCGCTCAACCTAC AGGACCGAGACTATCCATTGA 52.6 47.6 58.1 57.7 711-712 bp 1.0 62.7
rps16-accD_p2 AACAAGGCGCTCAACCTAC GTCTTCTTCACTTACACCGGT 52.6 47.6 58.1 57.6 741-742 bp 1.0 62.1
rps16-accD_p3 GAACAAGGCGCTCAACCTAC AGGACCGAGACTATCCATTGA 55.0 47.6 59.2 57.7 712-713 bp 1.0 61.7
rps16-accD_p4 GAACAAGGCGCTCAACCTA AGGACCGAGACTATCCATTGA 52.6 47.6 57.8 57.7 712-713 bp 1.0 61.5
rps16-accD_p5 GAACAAGGCGCTCAACCTAC GTCTTCTTCACTTACACCGGT 55.0 47.6 59.2 57.6 742-743 bp 1.0 61.0