Hotspot

trnG-UCC-trnS-GCU

LSC · 52425-52846 · 422 bp

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Pi 0.022
MarkerSeek score 60.40
Primer available yes
Label trnG-UCC-trnS-GCU

Feature Metadata

label_name
trnG-UCC-trnS-GCU
feature_type
igs
region
LSC
length_bp
422
variable_sites
9
pi
0.022
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.97
markerseek_score
60.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnG-UCC-trnS-GCU_p1 GGGGTCCATAGAACGAATCG CAATCCGACGCTTTAGTCCA 55.0 50.0 57.8 58.0 497-503 bp 1.0 82.1
trnG-UCC-trnS-GCU_p2 GGGGTCCATAGAACGAATCG AATCCGACGCTTTAGTCCAC 55.0 50.0 57.8 58.0 496-502 bp 1.0 82.1
trnG-UCC-trnS-GCU_p3 GGTCCATAGAACGAATCGCA CAATCCGACGCTTTAGTCCA 50.0 50.0 57.8 58.0 495-501 bp 1.0 81.6
trnG-UCC-trnS-GCU_p4 GGTCCATAGAACGAATCGCA AATCCGACGCTTTAGTCCAC 50.0 50.0 57.8 58.0 494-500 bp 1.0 81.6
trnG-UCC-trnS-GCU_p5 GGGGTCCATAGAACGAATCG ACGGAAAGAGAGGGATTCGA 55.0 50.0 57.8 58.2 550-556 bp 1.0 80.6