Hotspot

atpH-atpF

LSC · 47823-48428 · 606 bp

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Pi 0.00839
MarkerSeek score 65.20
Primer available yes
Label atpH-atpF

Feature Metadata

label_name
atpH-atpF
feature_type
igs
region
LSC
length_bp
606
variable_sites
5
pi
0.00839
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.98
markerseek_score
65.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
atpH-atpF_p1 GGCTGGTTGTAGCATTAGCA CCAGTAGCCCAAACAAAGGA 50.0 50.0 58.2 57.7 695-720 bp 1.0 77.4
atpH-atpF_p2 TGGGCTGGTTGTAGCATTAG CCAGTAGCCCAAACAAAGGA 50.0 50.0 57.6 57.7 697-722 bp 1.0 76.1
atpH-atpF_p3 AGGCAGAGGGAAAAATACGG CCAGTAGCCCAAACAAAGGA 50.0 50.0 57.6 57.7 764-789 bp 1.0 75.9
atpH-atpF_p4 CCAGAGGCAGAGGGAAAAAT CCAGTAGCCCAAACAAAGGA 50.0 50.0 57.5 57.7 768-793 bp 1.0 75.1
atpH-atpF_p5 GCAGAGGGAAAAATACGGGG CCAGTAGCCCAAACAAAGGA 55.0 50.0 58.6 57.7 762-787 bp 1.0 74.4