Hotspot

trnE-UUC-psbM

LSC · 30859-31799 · 941 bp

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Pi 0.015
MarkerSeek score 56.30
Primer available yes
Label trnE-UUC-psbM

Feature Metadata

label_name
trnE-UUC-psbM
feature_type
igs
region
LSC
length_bp
941
variable_sites
14
pi
0.015
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
56.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnE-UUC-psbM_p1 TGTCCTGAACCACTAGACGA AGGAACTAGAATAAACAGTGCAGT 50.0 37.5 57.7 57.9 1022-1039 bp 1.0 46.3
trnE-UUC-psbM_p2 CCTGAACCACTAGACGATGG AGGAACTAGAATAAACAGTGCAGT 55.0 37.5 57.4 57.9 1019-1036 bp 1.0 43.9
trnE-UUC-psbM_p3 CTGAACCACTAGACGATGGG AGGAACTAGAATAAACAGTGCAGT 55.0 37.5 57.4 57.9 1018-1035 bp 1.0 43.9
trnE-UUC-psbM_p4 TGTCCTGAACCACTAGACGA GGAACTAGAATAAACAGTGCAGT 50.0 39.1 57.7 56.5 1021-1038 bp 1.0 43.6
trnE-UUC-psbM_p5 TGTCCTGAACCACTAGACGA AGTAAAAAGGCAGTAGGAACT 50.0 38.1 57.7 54.5 1036-1053 bp 1.0 42.9