Hotspot

psbD-trnT-GGU

LSC · 28555-29761 · 1207 bp

Back to Grona

Pi 0.011
MarkerSeek score 59.80
Primer available yes
Label psbD-trnT-GGU

Feature Metadata

label_name
psbD-trnT-GGU
feature_type
igs
region
LSC
length_bp
1207
variable_sites
13
pi
0.011
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.98
markerseek_score
59.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbD-trnT-GGU_p1 CACAAAAACGAAACGGTCCC GTGGTAGAGTAACGCCATGG 50.0 55.0 58.2 58.1 1358-1362 bp 1.0 80.3
psbD-trnT-GGU_p2 CCACAAAAACGAAACGGTCC GTGGTAGAGTAACGCCATGG 50.0 55.0 58.2 58.1 1359-1363 bp 1.0 80.3
psbD-trnT-GGU_p3 CCCACAAAAACGAAACGGTC GTGGTAGAGTAACGCCATGG 50.0 55.0 58.2 58.1 1360-1364 bp 1.0 80.3
psbD-trnT-GGU_p4 CACAAAAACGAAACGGTCCC TCAGTGGTAGAGTAACGCCA 50.0 50.0 58.2 58.1 1361-1365 bp 1.0 80.1
psbD-trnT-GGU_p5 CCACAAAAACGAAACGGTCC TCAGTGGTAGAGTAACGCCA 50.0 50.0 58.2 58.1 1362-1366 bp 1.0 80.1