Hotspot

trnG-GCC-psbZ

LSC · 24676-25252 · 577 bp

Back to Grona

Pi 0.014
MarkerSeek score 55.40
Primer available yes
Label trnG-GCC-psbZ

Feature Metadata

label_name
trnG-GCC-psbZ
feature_type
igs
region
LSC
length_bp
577
variable_sites
8
pi
0.014
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
55.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnG-GCC-psbZ_p1 CGTCTTCTCCTTGGCAAAGA AGTGGGTATTCTTAATTCTCTCA 50.0 34.8 57.8 54.5 655-657 bp 1.0 41.6
trnG-GCC-psbZ_p2 CGTCTTCTCCTTGGCAAAGA AGTGGGTATTCTTAATTCTCTCAT 50.0 33.3 57.8 55.1 655-657 bp 1.0 41.6
trnG-GCC-psbZ_p3 CGTCTTCTCCTTGGCAAAGA GTGGGTATTCTTAATTCTCTCA 50.0 36.4 57.8 53.0 654-656 bp 1.0 41.6
trnG-GCC-psbZ_p4 CGTCTTCTCCTTGGCAAAGA AGTGGGTATTCTTAATTCTCTCATT 50.0 32.0 57.8 55.8 655-657 bp 1.0 41.6
trnG-GCC-psbZ_p5 CGTCTTCTCCTTGGCAAAGA AGTGGGTATTCTTAATTCTCTC 50.0 36.4 57.8 52.7 655-657 bp 1.0 41.6