Hotspot

trnT-UGU-rps4

LSC · 15264-15585 · 322 bp

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Pi 0.0219
MarkerSeek score 62.30
Primer available yes
Label trnT-UGU-rps4

Feature Metadata

label_name
trnT-UGU-rps4
feature_type
igs
region
LSC
length_bp
322
variable_sites
7
pi
0.0219
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
62.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnT-UGU-rps4_p1 CTCAGAGGTTAGAGCATCGC AGGTCCTCTGTAACGTGACA 55.0 50.0 57.9 58.0 406-409 bp 1.0 80.6
trnT-UGU-rps4_p2 ATGGTCATCGGTTCGAATCC AGGTCCTCTGTAACGTGACA 50.0 50.0 57.8 58.0 374-377 bp 1.0 79.9
trnT-UGU-rps4_p3 CGGTTATGTTAGCCCGCTTA AGGTCCTCTGTAACGTGACA 50.0 50.0 57.8 58.0 427-430 bp 1.0 79.8
trnT-UGU-rps4_p4 CTCAGAGGTTAGAGCATCGC CTGCCGGCCTTTTACTAGTT 55.0 50.0 57.9 57.9 467-470 bp 1.0 79.6
trnT-UGU-rps4_p5 CGGTTATGTTAGCCCGCTTA CTGCCGGCCTTTTACTAGTT 50.0 50.0 57.8 57.9 488-491 bp 1.0 78.8