Hotspot

trnK-UUU-rbcL

LSC · 4402-5226 · 825 bp

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Pi 0.0114
MarkerSeek score 55.90
Primer available yes
Label trnK-UUU-rbcL

Feature Metadata

label_name
trnK-UUU-rbcL
feature_type
igs
region
LSC
length_bp
825
variable_sites
9
pi
0.0114
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.96
markerseek_score
55.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rbcL_p1 AGAGTCGCTCTTAAAAGCCG GAGCCGTATTTGCCAGAGAA 50.0 50.0 58.0 58.0 1513-1543 bp 1.0 82.5
trnK-UUU-rbcL_p2 AGTCGCTCTTAAAAGCCGAG GAGCCGTATTTGCCAGAGAA 50.0 50.0 58.0 58.0 1511-1541 bp 1.0 82.5
trnK-UUU-rbcL_p3 GAGTCGCTCTTAAAAGCCGA GAGCCGTATTTGCCAGAGAA 50.0 50.0 58.0 58.0 1512-1542 bp 1.0 82.4
trnK-UUU-rbcL_p4 AGAGTCGCTCTTAAAAGCCG AAGAGCCGTATTTGCCAGAG 50.0 50.0 58.0 58.0 1515-1545 bp 1.0 82.4
trnK-UUU-rbcL_p5 AGTCGCTCTTAAAAGCCGAG AAGAGCCGTATTTGCCAGAG 50.0 50.0 58.0 58.0 1513-1543 bp 1.0 82.4