Hotspot

rpoC1

LSC · 21531-24322 · 2792 bp

Back to Griffitharia

Pi 0.00146

MarkerSeek score 35.70

Primer available yes

Label rpoC1

Feature Metadata

label_name: rpoC1
feature_type: gene
region: LSC
length_bp: 2792
variable_sites: 11
pi: 0.00146
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 35.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoC1_p1	GGTGAACTGGAGAAACCCAA	TTTGGCTCTGGAACTGAACC	50.0	50.0	57.6	58.0	2952-2964 bp	1.0	77.5
rpoC1_p2	GGTGAACTGGAGAAACCCAA	TTGCTCGTTCGAGAACTACG	50.0	50.0	57.6	58.0	2975-2987 bp	1.0	77.3
rpoC1_p3	GAAACCCAAAATGACACGGG	TTTGGCTCTGGAACTGAACC	50.0	50.0	57.6	58.0	2941-2953 bp	1.0	76.9
rpoC1_p4	GGTGAACTGGAGAAACCCAA	ACGATCTTTGGCTCTGGAAC	50.0	50.0	57.6	57.9	2958-2970 bp	1.0	76.8
rpoC1_p5	GAAACCCAAAATGACACGGG	TTGCTCGTTCGAGAACTACG	50.0	50.0	57.6	58.0	2964-2976 bp	1.0	76.7