Hotspot

ndhF

SSC · 113770-116025 · 2256 bp

Back to Griffitharia

Pi 0.00154

MarkerSeek score 52.40

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2256
variable_sites: 10
pi: 0.00154
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	TCTGGATGGGAATCAAGAACA	GAACCGGTGACTCAGAAACA	42.9	50.0	56.6	57.8	2352-2356 bp	1.0	59.5
ndhF_p2	TCTGGATGGGAATCAAGAACAA	GAACCGGTGACTCAGAAACA	40.9	50.0	57.3	57.8	2352-2356 bp	1.0	57.3
ndhF_p3	TGGATGGGAATCAAGAACAAGA	GAACCGGTGACTCAGAAACA	40.9	50.0	57.3	57.8	2350-2354 bp	1.0	57.3
ndhF_p4	TCTGGATGGGAATCAAGAACA	AGAACCGGTGACTCAGAAAC	42.9	50.0	56.6	57.5	2353-2357 bp	1.0	57.2
ndhF_p5	TCTGGATGGGAATCAAGAACA	AACCGGTGACTCAGAAACAA	42.9	45.0	56.6	57.3	2351-2355 bp	1.0	55.8