Hotspot

clpP

LSC · 73030-75057 · 2028 bp

Back to Griffitharia

Pi 0.00141

MarkerSeek score 54.50

Primer available yes

Label clpP

Feature Metadata

label_name: clpP
feature_type: gene
region: LSC
length_bp: 2028
variable_sites: 10
pi: 0.00141
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP_p1	TCTCAAATCACCAATTCGTCTGA	ACGCTTTCACATCAAAGTGA	39.1	40.0	58.1	55.9	2119-2136 bp	1.0	40.6
clpP_p2	CTCAAATCACCAATTCGTCTGA	ACGCTTTCACATCAAAGTGA	40.9	40.0	56.7	55.9	2118-2135 bp	1.0	40.6
clpP_p3	TCTCAAATCACCAATTCGTCTG	ACGCTTTCACATCAAAGTGA	40.9	40.0	56.7	55.9	2119-2136 bp	1.0	40.6
clpP_p4	TCTCAAATCACCAATTCGTCTGA	TCCATTGTTACGCTTTCACA	39.1	40.0	58.1	55.6	2128-2145 bp	1.0	40.6
clpP_p5	TCTCAAATCACCAATTCGTCTGA	CCATTGTTACGCTTTCACATCA	39.1	40.9	58.1	57.6	2127-2144 bp	1.0	40.6