Hotspot

rpl32-trnL-UAG

SSC · 117246-118687 · 1442 bp

Back to Griffitharia

Pi 0.0028

MarkerSeek score 57.50

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 1442
variable_sites: 13
pi: 0.0028
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 57.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	GGGCAGCATTGAAAGCTTTT	AGCGTGTCTACCGATTTCAC	45.0	50.0	57.8	58.0	1530-1626 bp	1.0	81.6
rpl32-trnL-UAG_p2	GGGCAGCATTGAAAGCTTTT	CAGCGTGTCTACCGATTTCA	45.0	50.0	57.8	58.0	1531-1627 bp	1.0	81.6
rpl32-trnL-UAG_p3	TAGGGCAGCATTGAAAGCTT	AGCGTGTCTACCGATTTCAC	45.0	50.0	57.8	58.0	1532-1628 bp	1.0	81.2
rpl32-trnL-UAG_p4	TAGGGCAGCATTGAAAGCTT	CAGCGTGTCTACCGATTTCA	45.0	50.0	57.8	58.0	1533-1629 bp	1.0	81.2
rpl32-trnL-UAG_p5	GGGCAGCATTGAAAGCTTTT	TCCTAAGAGCAGCGTGTCTA	45.0	50.0	57.8	57.9	1540-1636 bp	1.0	80.6