Hotspot

trnT-UGU-trnL-UAA

LSC · 48920-50132 · 1213 bp

Back to Griffitharia

Pi 0.00238
MarkerSeek score 50.00
Primer available yes
Label trnT-UGU-trnL-UAA

Feature Metadata

label_name
trnT-UGU-trnL-UAA
feature_type
igs
region
LSC
length_bp
1213
variable_sites
9
pi
0.00238
unique_haplotype_count
6
primer_available
yes
alignment_reliability
1.00
markerseek_score
50.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnT-UGU-trnL-UAA_p1 GCGATGCTCTAACCTCTGAG ACCAATTTCGCCATATCCCC 55.0 50.0 57.9 58.0 1222-1270 bp 1.0 82.3
trnT-UGU-trnL-UAA_p2 GCGATGCTCTAACCTCTGAG TAGCGTCTACCAATTTCGCC 55.0 50.0 57.9 58.1 1230-1278 bp 1.0 81.7
trnT-UGU-trnL-UAA_p3 GGAATCGAACCGATGACCAT ACCAATTTCGCCATATCCCC 50.0 50.0 57.8 58.0 1254-1302 bp 1.0 81.3
trnT-UGU-trnL-UAA_p4 GGAATCGAACCGATGACCAT TAGCGTCTACCAATTTCGCC 50.0 50.0 57.8 58.1 1262-1310 bp 1.0 80.8
trnT-UGU-trnL-UAA_p5 GCGATGCTCTAACCTCTGAG AGTCCGTAGCGTCTACCAAT 55.0 50.0 57.9 58.2 1236-1284 bp 1.0 80.4