Hotspot

trnC-GCA-petN

LSC · 28844-29778 · 935 bp

Back to Griffitharia

Pi 0.00306

MarkerSeek score 54.40

Primer available yes

Label trnC-GCA-petN

Feature Metadata

label_name: trnC-GCA-petN
feature_type: igs
region: LSC
length_bp: 935
variable_sites: 9
pi: 0.00306
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-petN_p1	TTTCCCCAGTTCAAATCCGG	CAGCCCAAGCGAGATTTACT	50.0	50.0	58.1	58.0	972-1008 bp	1.0	81.5
trnC-GCA-petN_p2	GTATCATTTTGGCGGCATGG	CAGCCCAAGCGAGATTTACT	50.0	50.0	57.9	58.0	1024-1060 bp	1.0	81.0
trnC-GCA-petN_p3	TTTCCCCAGTTCAAATCCGG	AGTCCACTCCTTCCCCATAC	50.0	55.0	58.1	58.1	1030-1066 bp	1.0	80.7
trnC-GCA-petN_p4	TTTCCCCAGTTCAAATCCGG	GTCCACTCCTTCCCCATACT	50.0	55.0	58.1	58.1	1029-1065 bp	1.0	80.7
trnC-GCA-petN_p5	GTATCATTTTGGCGGCATGG	GTCCACTCCTTCCCCATACT	50.0	55.0	57.9	58.1	1081-1117 bp	1.0	80.1