Hotspot

trnH-GUG-psbA

LSC · 127-389 · 263 bp

Back to Griffitharia

Pi 0.0182

MarkerSeek score 55.70

Primer available yes

Label trnH-GUG-psbA

Feature Metadata

label_name: trnH-GUG-psbA
feature_type: igs
region: LSC
length_bp: 263
variable_sites: 14
pi: 0.0182
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 55.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	GATCCACTTGGCTACATCCG	GCGCTAACCTTGGTATGGAA	55.0	50.0	58.1	58.0	372-383 bp	1.0	83.4
trnH-GUG-psbA_p2	CAATCCACTGCCTTGATCCA	GCGCTAACCTTGGTATGGAA	50.0	50.0	57.9	58.0	386-397 bp	1.0	83.2
trnH-GUG-psbA_p3	ATATTATGGGCGAACGACGG	CCTCTAGACCTAGCTGCTGT	50.0	55.0	57.9	57.9	384-394 bp	0.875	78.0
trnH-GUG-psbA_p4	ATATTATGGGCGAACGACGG	TTCCCTCTAGACCTAGCTGC	50.0	55.0	57.9	57.9	387-397 bp	0.875	77.9
trnH-GUG-psbA_p5	ATATTATGGGCGAACGACGG	GCGCTAACCTTGGTATGGAA	50.0	50.0	57.9	58.0	434-444 bp	0.875	77.8