Hotspot

rpl16

LSC · 83838-85347 · 1510 bp

Back to Grewia

Pi 0.00309
MarkerSeek score 46.40
Primer available yes
Label rpl16

Feature Metadata

label_name
rpl16
feature_type
gene
region
LSC
length_bp
1510
variable_sites
7
pi
0.00309
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
46.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl16_p1 ATCGGCGATTACTAGCTCCT TAGGCCACGAAAAATCGAGG 50.0 50.0 58.1 58.0 2286-2288 bp 1.0 79.6
rpl16_p2 ATCGGCGATTACTAGCTCCT GTGGAGGGAATTGCACGTAT 50.0 50.0 58.1 58.0 2378-2380 bp 1.0 79.4
rpl16_p3 ACTGCTTCCTTGATCACAGC TAGGCCACGAAAAATCGAGG 50.0 50.0 58.2 58.0 2332-2334 bp 1.0 78.8
rpl16_p4 CTGCTTCCTTGATCACAGCA TAGGCCACGAAAAATCGAGG 50.0 50.0 58.2 58.0 2331-2333 bp 1.0 78.8
rpl16_p5 ATCGGCGATTACTAGCTCCT GTGTCGAATGGATCCGAGAG 50.0 55.0 58.1 57.9 2023-2025 bp 1.0 78.8