Hotspot

psbI

LSC · 7904-8014 · 111 bp

Back to Grewia

Pi 0.012
MarkerSeek score 59.90
Primer available yes
Label psbI

Feature Metadata

label_name
psbI
feature_type
gene
region
LSC
length_bp
111
variable_sites
2
pi
0.012
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbI_p1 CTTGGTGTCAAAATGTGTGGT TCGAATCCCTCTCTTTCCGT 42.9 50.0 57.2 58.2 293 bp 1.0 64.9
psbI_p2 CTTGGTGTCAAAATGTGTGGT AATGAAGCAAGGAACTCCCC 42.9 50.0 57.2 57.8 216 bp 1.0 64.6
psbI_p3 CTTGGTGTCAAAATGTGTGGT GAAGCAAGGAACTCCCCTTT 42.9 50.0 57.2 57.7 213 bp 1.0 64.0
psbI_p4 TTGGTGTCAAAATGTGTGGT TCGAATCCCTCTCTTTCCGT 40.0 50.0 56.0 58.2 292 bp 1.0 63.1
psbI_p5 TCTTGGTGTCAAAATGTGTGG TCGAATCCCTCTCTTTCCGT 42.9 50.0 56.9 58.2 294 bp 1.0 62.7