Hotspot

psaC

SSC · 124444-124689 · 246 bp

Pi 0.0108

MarkerSeek score 37.70

Primer available yes

Label psaC

Feature Metadata

label_name: psaC
feature_type: gene
region: SSC
length_bp: 246
variable_sites: 4
pi: 0.0108
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 37.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaC_p1	TGATGTTCAAAAATTTATAGATCCC	CAGAAAAACCCGTGCTCAAA	28.0	45.0	53.9	57.1	386-388 bp	1.0	41.2
psaC_p2	TGATGTTCAAAAATTTATAGATCCC	GTGGGGTTTTCTGGAACGTA	28.0	50.0	53.9	57.7	291 bp	1.0	41.1
psaC_p3	TGATGTTCAAAAATTTATAGATCCC	AAGTGGGGTTTTCTGGAACG	28.0	50.0	53.9	58.3	293 bp	1.0	41.1
psaC_p4	ATGATGTTCAAAAATTTATAGATCCC	GTGGGGTTTTCTGGAACGTA	26.9	50.0	54.4	57.7	292 bp	1.0	41.1
psaC_p5	ATGATGTTCAAAAATTTATAGATCCC	AAGTGGGGTTTTCTGGAACG	26.9	50.0	54.4	58.3	294 bp	1.0	41.1