Hotspot

ndhD

SSC · 124831-126333 · 1503 bp

Pi 0.000887

MarkerSeek score 32.90

Primer available yes

Label ndhD

Feature Metadata

label_name: ndhD
feature_type: gene
region: SSC
length_bp: 1503
variable_sites: 2
pi: 0.000887
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 32.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD_p1	CAAATTGCTTCCGCTCCAAG	TTGGACCGTATTCGCGATTT	50.0	45.0	58.0	58.0	2264-2267 bp	1.0	80.3
ndhD_p2	AGCAGCTATCGGATCAGCTA	TTGGACCGTATTCGCGATTT	50.0	45.0	58.1	58.0	2724-2727 bp	1.0	79.7
ndhD_p3	CAAATTGCTTCCGCTCCAAG	TTACTTGGACCGTATTCGCG	50.0	50.0	58.0	58.1	2268-2271 bp	1.0	79.7
ndhD_p4	AAATTGCTTCCGCTCCAAGA	TTGGACCGTATTCGCGATTT	45.0	45.0	58.1	58.0	2263-2266 bp	1.0	79.7
ndhD_p5	AATTGCTTCCGCTCCAAGAA	TTGGACCGTATTCGCGATTT	45.0	45.0	58.1	58.0	2262-2265 bp	1.0	79.7