Hotspot

trnW-CCA-trnP-UGG

LSC · 69291-69481 · 191 bp

Back to Grewia

Pi 0.0105
MarkerSeek score 57.20
Primer available yes
Label trnW-CCA-trnP-UGG

Feature Metadata

label_name
trnW-CCA-trnP-UGG
feature_type
igs
region
LSC
length_bp
191
variable_sites
3
pi
0.0105
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
57.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnW-CCA-trnP-UGG_p1 GTTTTGGAGACCCACGTTCT GGGTACAAAATGTCACGGGT 50.0 50.0 58.0 58.1 270 bp 1.0 79.8
trnW-CCA-trnP-UGG_p2 GTTTTGGAGACCCACGTTCT TGGGTACAAAATGTCACGGG 50.0 50.0 58.0 58.1 271 bp 1.0 79.8
trnW-CCA-trnP-UGG_p3 GTTTTGGAGACCCACGTTCT GTCACGGGTTCAAATCCTGT 50.0 50.0 58.0 58.1 259 bp 1.0 79.8
trnW-CCA-trnP-UGG_p4 GTTTTGGAGACCCACGTTCT CACGGGTTCAAATCCTGTCA 50.0 50.0 58.0 58.1 257 bp 1.0 79.8
trnW-CCA-trnP-UGG_p5 GTTTTGGAGACCCACGTTCT TGTCACGGGTTCAAATCCTG 50.0 50.0 58.0 58.1 260 bp 1.0 79.8